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Rapidly measured indicators of recreational water quality and swimming-associated illness at marine beaches: a prospective cohort study

Timothy J Wade1*, Elizabeth Sams1, Kristen P Brenner2, Richard Haugland2, Eunice Chern2, Michael Beach3, Larry Wymer2, Clifford C Rankin2, David Love4, Quanlin Li1, Rachel Noble5 and Alfred P Dufour2

Author Affiliations

1 United States Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Research Triangle Park, North Carolina, USA

2 United States Environmental Protection Agency, National Exposure Research Laboratory, Cincinnati, Ohio, USA

3 Centers for Disease Control and Prevention, Division of Foodborne, Waterborne and Environmental Diseases, Atlanta, Georgia, USA

4 Johns Hopkins University, Baltimore, Maryland, USA

5 University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

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Environmental Health 2010, 9:66  doi:10.1186/1476-069X-9-66

Published: 31 October 2010

Abstract

Introduction

In the United States and elsewhere, recreational water quality is monitored for fecal indicator bacteria to help prevent swimming-associated illnesses. Standard methods to measure these bacteria take at least 24 hours to obtain results. Molecular approaches such as quantitative polymerase chain reaction (qPCR) can estimate these bacteria faster, in under 3 hours. Previously, we demonstrated that measurements of the fecal indicator bacteria Enterococcus using qPCR were associated with gastrointestinal (GI) illness among swimmers at freshwater beaches. In this paper, we report on results from three marine beach sites.

Methods

We interviewed beach-goers and collected water samples at marine beaches affected by treated sewage discharges in Mississippi in 2005, and Rhode Island and Alabama in 2007. Ten to twelve days later, we obtained information about gastrointestinal, respiratory, eye, ear and skin symptoms by telephone. We tested water samples for fecal indicator organisms using qPCR and other methods.

Results

We enrolled 6,350 beach-goers. The occurrence of GI illness among swimmers was associated with a log10-increase in exposure to qPCR-determined estimates of fecal indicator organisms in the genus Enterococcus (AOR = 2.6, 95% CI 1.3-5.1) and order Bacteroidales (AOR = 1.9, 95% CI 1.3-2.9). Estimates of organisms related to Clostridium perfringens and a subgroup of organisms in the genus Bacteroides were also determined by qPCR in 2007, as was F+ coliphage, but relationships between these indicators and illness were not statistically significant.

Conclusions

This study provides the first evidence of a relationship between gastrointestinal illness and estimates of fecal indicator organisms determined by qPCR at marine beaches.