Figure 6.

Expression of catecholaminergic neuronal markers in N2a-Rα cells. The expression of marker of catecholaminergic neuron, namely TH and Nurr is examined. The expression of TH mRNA and Nurr mRNA in the N2a-Rα cells and N2a-Vc cells were measured by RT-PCR with specific primers for TH and Nurr. Approximately twice the amount of TH mRNA was detected in the N2a-Rα cells compared with the N2a-Vc cells. Nurr mRNA was also detected significantly. Data were normalized to the expression level of cyclophilin mRNA and presented relative to the value of N2a-Vc. Values are presented as the mean ± S. D. of four replicate determinations; **p < 0.01 and *p < 0.05