Table 1

LPS induces GM-SCF, G-CSF, LIF, and IL-6 mRNAs in bone marrow stromal cells
Gene
 N
 Mean Fold Changea ± SE
 P-valueb
IL-11
 4
 1.3 ± 0.2
 0.27
IL-7
 5
 0.9 ± 0.2
 0.58
GM-CSF
 3
 Increasedc
 -
G-CSF
 4
 Increasedc
 -
LIF
 5
 5.7 ± 1.2
 0.02*
IL-6
 7
 7.0 ± 1.5
 0.01*
SCF
 4
 1.1 ± 0.1
 0.40
M-CSF
 5
 1.0 ± 0.1
 0.79
TGF-β1
 2
 1.2, 1.4
 0.26
TGF-β3
 2
 0.8, 0.9
 0.18
MIF
 2
 0.9, 1.0
 0.52

a From the Ribonuclease Protection Assays (RPAs), mean fold-change was determined by normalizing the optical density band of the LPS-treated sample to the housekeeping gene GAPDH or L-32, then expressing it relative to the optical density of the normalized band from the vehicle-treated sample. b Based on one-sample t-test to determine whether mean was significantly different from maximum LPS induction (μ = 1.0). c Expression of GM-CSF and G-CSF was sometimes undetectable in unstimulated cells, thus the LPS-induced expression could not be described appropriately as "fold change" * p < 0.05

Jensen et al. Environmental Health: A Global Access Science Source 2003 2:16   doi:10.1186/1476-069X-2-16

Open Data